Three Sisters (Australia), a rock formation near Katoomba, NSW
The Three Sisters (Queensland), three islands
Three Sisters Island (Tasmania), three small islands
Canada
The Three Sisters (Alberta), three peaks in the Canadian Rockies near Canmore, Alberta
The Three Sisters (Lake Huron), three islands in Lake Huron
The Three Sisters (Temagami), three lakes in Temagami, Ontario
Three Sisters (Elk Valley), three peaks in the Canadian Rockies just north of the town of Fernie, British Columbia
Three Sisters Range, three peaks in the Stikine Country, British Columbia
Three Sisters Lakes Provincial Park, a park near Hixon, British Columbia
Three Sisters, a rock formation along the shore of Cape Chignecto Provincial Park
Ireland
The Three Sisters (Ireland), three rivers
An Triúr Deirféar (Irish for The Three Sisters), three peaks on the Dingle Peninsula
South Africa
Three Sisters (Northern Cape), a rock formation consisting of a group of three conical peaks situated on the watershed between Beaufort West and Richmond
United Kingdom
Three Sisters (Glen Coe), three ridges
Three Sisters Recreation Area, Wigan
United States
Three Sisters, mineral springs at Lake Ouachita State Park, Arkansas
Three Sisters (Los Angeles County), a mountain range in California
Three Sisters (Riverside County), a mountain range in California
Three Sisters (Siskiyou County), a mountain range in California
Three Sisters (District of Columbia), three islands
Three Sisters Springs (Florida)
Three Sisters (Georgia), three peaks
Three Sisters (Oregon), three volcanoes
Three Sisters Wilderness, Oregon
Three Sisters (Pittsburgh), three bridges in Pennsylvania
Stage, film and television
Three Sisters (play), a play by Anton Chekhov
The Three Sisters (1966 film), an American film
Three Sisters (1970 film), a British film directed by Laurence Olivier
The Three Sisters (1970 film), a British television adaptation
Three Sisters (1988 film), aka Love and Fear, directed by Margarethe von Trotta
Three Sisters (1994 film), a Russian film adaptation
Three Sisters (opera), a 1998 opera by Péter Eötvös
The Three Sisters (fairy tale), an Italian fairy tale
The Three Sisters (1930 film), an American film directed by Paul Sloane
Three Sisters (musical), a 1934 musical by Jerome Kern and Oscar Hammerstein II
Three Sisters (1934 film), a Chinese film of the 1930s
Three Sisters (2012 film), a Chinese documentary film
Three Sisters (2020 film) a 2020 South Korean film
Three Sisters (American TV series), a 2001–2002 American TV series starring Dyan Cannon, Katherine LaNasa, and David Alan Basche
Three Sisters (South Korean TV series), a 2010 South Korean television drama
Mythology
Gorgons, three sisters in Greek mythology, named Stheno, Euryale, and Medusa
Graeae, three grey sisters (witches) in Greek mythology, named Deino, Enyo, and Pemphredo
Other uses
Three Sisters (album), a 2004 album by Alice Donut
Three Sisters (agriculture), a Native American agricultural technique for planting maize (corn), beans, and squash close together for shared benefits
Three Sisters (sternwheeler), a steamboat
Three Sisters tomato, a variety of tomato
Three Sisters Bridge, a cancelled bridge over the Potomac River in Washington, D.C.
Three Sisters Tavern, a restaurant in Portland, Oregon
Three Sisters of Nauset, a trio of lighthouses in Nauset, Massachusetts
Three sisters, a name for a combination of rogue waves, particularly on Lake Superior
"The Three Sisters" is a colloquial phrase referring to the Dutch Reformed Church in South Africa (NGK), Dutch Reformed Church in South Africa (NHK), and Reformed Churches in South Africa
Orion's Belt, a part of the Orion constellation, or group of stars, located on the celestial equator